Course detail
Experimental Biophotonics
FSI-TEB-A Acad. year: 2020/2021 Winter semester
Basics of cell biology in vitro: structure and functions of the cell; isolation into and maintenance of cells from multicellular organism into in vitro systems; mastering the specialty of preparation of live cells for microscopical observations. Protocols of in vitro investigations of cell behaviour. Interpretation and evaluation of results of microscopical observations of cell behaviour. The role of light microscopy techniques for study of live cells in vitro: principles of light microscopy, fluorescent and confocal microscopy, quantitative phase contrast in holographic microscopy, image processing and analysis.
Language of instruction
English
Number of ECTS credits
3
Supervisor
Department
Learning outcomes of the course unit
Acquaintance with the basics of live cell biology in vitro; preparation and cultivation of cell for microscopy observation; application of light microscopies to study cell living in vitro; elements of interpretation of results of biological experiments from physical and biological points of view.
Understanding problems and gaining insight into current trends of biological microscopy.
Acquiring of widened ability to interdisciplinary professional activity.
Prerequisites
Knowledge of elements of geometric optics.
Planned learning activities and teaching methods
The course is taught through lectures explaining the basic principles and theory of the discipline. Teaching is suplemented by practical laboratory work.
Assesment methods and criteria linked to learning outcomes
Student assesment will take into consideration his/her work at laboratory and wiil depend on the result of final discussion on specified themes. Notes and literature is permitted.
Aims
Objective of the theme is the introduction into the basics and prospective of live cell biology in vitro as observed and studied with techniques of light microscopy.
Specification of controlled education, way of implementation and compensation for absences
Presence in all teaching units is required because of their mutual links. In the case of student absention, tacher will specify alternative requirements.
The study programmes with the given course
Programme N-FIN-P: Physical Engineering and Nanotechnology, Master's, elective
Programme M2A-P: Applied Sciences in Engineering, Master's
branch M-PMO: Precise Mechanics and Optics, elective
Type of course unit
Lecture
26 hours, optionally
Syllabus
Structure and functions of the cell
Cells isolation into in vitro systems
Principles of light microscopy
Mastering the specialty of preparation of live cells for microscopical observations
Protocols of in vitro investigations of cell behaviour.
Fluorescence and confocal microscopy
Quantitative phase contrast in holographic microscopy
Image processing and analysis
Analysis of results of biological experiments
Laboratory exercise
13 hours, compulsory
Syllabus
Lecture themes are developed by laboratory demonstrations and laboratory work of students.