Course detail
Microscopy and Spectroscopy
FSI-TMK Acad. year: 2022/2023 Winter semester
Introduction to light microscopy (historical overview completed by substantial pieces of knowledge of geometric and wave optics, light-microscope optical setup, basic techniques of light microscopy and practical knowledge), theoretical description of image formation (wave theory of image formation based on the Abbe theory), confocal microscopy (principle, setup of the device, imaging properties), fluorescence microscopy (principle, setup of the device, imaging properties, methods), interference and holographic microscopy (principle, setup of the device, imaging properties), spectroscopic methods, X-ray photoelectron spectroscopy (XPS, principle, setup of the device, parameters), secondary ion mass spectrometry (SIMS, principle, setup of the device, parameters), low-energy ion scattering spectroscopy (LEIS, principle, setup of the device, parameters).
Demonstrations and practical exercises on light microscopy and spectroscopy and on particle spectroscopy are carried out in laboratories.
Language of instruction
Czech
Number of ECTS credits
5
Supervisor
Department
Learning outcomes of the course unit
Students will learn about the history and modern techniques and approaches in the field of light microscopy and spectroscopies (light and particle) and fundamental practical experience with relevant devices. Among others, it help them to select their own topic (for diploma or doctoral thesis).
Prerequisites
Elementary Physics, Quantum Physics, Solid State Physics, Surfaces and Thin Films, Geometrical and wave optics.
Planned learning activities and teaching methods
The course is taught through lectures explaining the basic principles and theory of the discipline. Teaching is suplemented by practical laboratory work.
Assesment methods and criteria linked to learning outcomes
The assessment of a student is made upon his performance in written and oral part of the exam.
Aims
The goal of the course is to provide students an overview of the principle and methods of light microscopy and spectroscopy, and of particle spectroscopy and give them practical experience with relevant devices.
Specification of controlled education, way of implementation and compensation for absences
The presence of students is monitored by the tutor. Maximum of tolerated absence is 25 %.
The study programmes with the given course
Programme N-FIN-P: Physical Engineering and Nanotechnology, Master's, compulsory
Programme N-PMO-P: Precise Mechanics and Optics, Master's, compulsory
Type of course unit
Lecture
26 hours, optionally
Teacher / Lecturer
Syllabus
Introduction to microscopy
Theory of imaging
Confocal microscopy
Fluorescence microscopy
Interference and holographic microscopy
Spectroscopic methods
X-ray photoelectron spectroscopy (XPS)
Secondary ion mass spectrometry (SIMS)
Low-energy ion scattering spectroscopy (LEIS)
Laboratory exercise
13 hours, compulsory
Teacher / Lecturer
Syllabus
The calculations of supportive theoretical examples take place during the whole semester. Demonstrations and practical exercises in laboratory of optical microscopy, in laboratory of surfaces and thin films.