Course detail
Experimental Biophotonics
FSI-TEB-A Acad. year: 2023/2024 Winter semester
Basics of cell biology in vitro: structure and functions of the cell; isolation of cells from multicellular organisms and their maintenance in vitro; mastering the specialty of preparation of live cells for microscopical observations. Protocols of in vitro investigations of cell behaviour. Interpretation and evaluation of results of microscopical observations of cell behaviour. The role of light microscopy techniques for study of live cells in vitro: principles of light microscopy, fluorescence and confocal microscopy, quantitative phase imaging in holographic microscopy, image processing and analysis.
Demonstrations and practical sections in the laboratory.
Language of instruction
English
Number of ECTS credits
3
Supervisor
Department
Entry knowledge
Knowledge of elements of geometric optics.
Rules for evaluation and completion of the course
Student assesment will take into consideration his/her work at laboratory and wiil depend on the result of final discussion on specified themes. Notes and literature is permitted.
Presence in all teaching units is required because of their mutual links. In the case of student absention, tacher will specify alternative requirements.
Aims
Objective of the theme is the introduction into the basics and prospective of live cell biology in vitro as observed and studied with techniques of light microscopy.
Acquaintance with the basics of live cell biology in vitro; preparation and cultivation of cell for microscopy observation; application of light microscopies to study cell living in vitro; elements of interpretation of results of biological experiments from physical and biological points of view.
Understanding problems and gaining insight into current trends of biological microscopy.
Acquiring of widened ability to interdisciplinary professional activity.
The study programmes with the given course
Programme N-FIN-P: Physical Engineering and Nanotechnology, Master's, elective
Programme N-PMO-P: Precise Mechanics and Optics, Master's, elective
Type of course unit
Lecture
13 hours, optionally
Syllabus
Structure and functions of the cell
Cells isolation into in vitro systems
Principles of light microscopy
Mastering the specialty of preparation of live cells for microscopical observations
Protocols of in vitro investigations of cell behaviour.
Fluorescence and confocal microscopy
Quantitative phase contrast in holographic microscopy
Image processing and analysis
Analysis of results of biological experiments
Laboratory exercise
13 hours, compulsory
Syllabus
Lecture themes are developed by laboratory demonstrations and laboratory work of students.