Course detail
Experimental Biophotonics
FSI-TEB-A Acad. year: 2024/2025 Winter semester
Basics of biophotonics including principles of light microscopy, fluorescence, confocal microscopy, quantitative phase imaging especially in holographic microscopy, and polarization microscopy. Basics of cell biology in vitro: structure and functions of the cell; isolation of cells from multicellular organisms and their maintenance in vitro; mastering the specialty of preparation of live cells for microscopy observations. Protocols of in vitro investigations of cell behavior. The role of light microscopy techniques for the study of live cells in vitro:
Applications in neurosciences and cancer research.
Demonstrations and practical sections in the laboratory.
Language of instruction
English
Number of ECTS credits
3
Supervisor
Department
Entry knowledge
Knowledge of elements of geometric optics.
Rules for evaluation and completion of the course
Student assessment will take into consideration his/her work at the laboratory and will depend on the result of the final discussion.
Presence in all teaching units is required because of their mutual links. In the case of student absence, the teacher will specify alternative requirements.
Aims
The objective of this theme is the introduction of biophotonics including light microscopy techniques for biology. The theme also includes the basics of cell biology and the application of light microscopy in studies of live cells in vitro, and example applications in neurosciences and cancer research.
Acquiring of widened ability to interdisciplinary professional activity.
The study programmes with the given course
Programme N-FIN-P: Physical Engineering and Nanotechnology, Master's, elective
Programme N-PMO-P: Precise Mechanics and Optics, Master's, elective
Type of course unit
Lecture
13 hours, optionally
Syllabus
Basic principles of light microscopy for biology
Cell biology and preparation of samples for microscopy
Interferometry and holographic microscopy
Fluorescence, confocal and 2-photon microscopy
Applications in tumor and cell biology
Polarization microscopy and imaging of extracellular matrix
Laboratory exercise
13 hours, compulsory
Syllabus
Lecture themes are developed by laboratory demonstrations and laboratory work of students.