Course detail
Advanced Light Microscopy - Imaging Theory
FSI-9MIA Acad. year: 2024/2025 Summer semester
The course explains principles of light microscopy of three-dimensional objects in terms of wave optics. Image properties are described using the formalism of 3D pupil and transfer functions. Properties of advanced microscopic techniques for 3D imaging are explained, especially those of confocal microscopy and coherence-controlled holographic microscopy.
Language of instruction
Czech
Supervisor
Department
Entry knowledge
Wave and Fourier optics, geometrical optics, fundamentals of light microscopy.
Rules for evaluation and completion of the course
Examination is oral. Detailed knowledge of explained matter is proved. Examined student can use a literature.
Presence at lectures is not obligatory, but recommended.
Aims
Understanding of principles of 3D light microscopy and microscopic imaging.
Acquiring of methods for fundamental 3D imaging characteristics calculation.
Knowledge of fundamentals of theoretical description of microscope imaging of 3D objects of their relation to experiment and competence to apply them in practice.
The study programmes with the given course
Programme D-FIN-K: Physical Engineering and Nanotechnology, Doctoral, recommended course
Programme D-FIN-P: Physical Engineering and Nanotechnology, Doctoral, recommended course
Type of course unit
Lecture
20 hours, optionally
Syllabus
1. Important knowledge in the field of light microscopy and wave optics
2. Light wave interaction with an object
3. Imaging from the point of view of wave optics
4. Imaging by confocal micorscope
5. Imaging by interference and holographic microscope
6. Imaging by fluorescence microscope
7. Imaging in bright field
8. Another specific cases of imaging