Course detail
Biophotonics
FSI-TBF Acad. year: 2024/2025 Winter semester
Basics of biophotonics including principles of light microscopy, fluorescence, confocal microscopy, quantitative phase imaging especially in holographic microscopy, polarisation microscopy, fiber optics and endoscopes, optical micromanipulations, and photopolymerization of nanostructures. Basics of cell biology in vitro: structure and functions of the cell; isolation of cells from multicellular organisms and their maintenance in vitro; mastering the specialty of preparation of live cells for microscopy observations. Protocols of in vitro investigations of cell behavior. The role of light microscopy techniques for the study of live cells in vitro:
Applications in neurosciences and cancer research.
Demonstrations and practical sections in the laboratory.
Language of instruction
Czech
Number of ECTS credits
2
Supervisor
Department
Entry knowledge
Knowledge of elements of geometric optics.
Rules for evaluation and completion of the course
Student assessment will take into consideration his/her work at the laboratory and will depend on his/her presentation of selected relevant scientific publication during a colloquium organized at the end.
Presence in all teaching units is recommended because of their mutual links.
Aims
The objective of the theme is the introduction of biophotonics, light microscopy techniques for biology, fiber optics and endoscopes, optical micromanipulation, and polymerization of nanostructures. The theme also includes the basics of cell biology and the application of light microscopy in studies of live cells in vitro, and example applications in neurosciences and cancer research.
Acquiring of widened ability to interdisciplinary professional activity.
The study programmes with the given course
Programme B-FIN-P: Physical Engineering and Nanotechnology, Bachelor's, compulsory-optional
Type of course unit
Lecture
13 hours, optionally
Syllabus
Basic principles of light microscopy for biology
Cell biology and preparation of samples for microscopy
Interferometry and holographic microscopy
Fluorescence, confocal and 2-photon microscopy
Fiber Optics, endoscopes, and applications in neurobiology
Applications in tumor and cell biology
Optical micromanipulation methods and optical tweezers
Photopolymerization of nanostructures
Polarization microscopy and imaging of extracellular matrix
Laboratory exercise
13 hours, compulsory
Syllabus
Tissue culture and preparation of cells
Holograhic interferometry
Confocal microscopy