Porovnání metod pro vyhodnocení depozice částic v modelu lidských plic

Comparison of methods for evaluation of aerosol deposition in the model of human lungs

conference paper

en

It seems to be very convenient to receive a medicine by inhalation instead of injection. Unfortunately transport of particles and targeted delivery of a drug in human respiratory airways is very complicated task. Therefore we carried out experiments and tested different methods for evaluation of particle deposition in a model of human lungs. The model included respiratory airways from oral cavity to 7th generation of branching. Particles were dispersed by TSI Small-scale Powder Disperser 3433 and delivered to the model. The model was disassembled into segments after the deposition of the particles and local deposition was measured. Two methods were used to analyse the samples, fluorescence spectroscopy and optical microscopy. The first method was based on measuring the intensity of luminescence, which represented the particle deposition. The second method used the optical microscope with phase-contrast objective. A dispersion of isopropanol and particles was filtrated using a vacuum filtration unit, a filter was placed on glass slide and made transparent. The particles on the filter were counted manually and the deposition was calculated afterwards. The results of the methods were compared and both methods proved to be useful.

Podávání léků inhalací místo injekční cestou je velice pohodlné. Avšak transport aerosolů a jeho cílená dodávka v lidských dýchacích cestách je velice komplikovaný problém. Proto bylo provedeno několik depozičních experimentů a odzkoušeno několik metod pro vyhodnocení depozice v modelu lidských plic. Model obsahoval dýchací cesty od ústní dutiny až po 7. generaci větvení. Částice byly dispergovány pomocí TSI SSPD 3433 a aplikovány do modelu. Po ukončení depozice byl model rozebrán a depozice v jednotlivých částech byla analyzována. Pro vyhodnocení byly použity 2 metody, fluorescenční spektroskopie a optická mikroskopie. První metoda využívá měření intenzity fluorescence částic, jejíž velikost určuje míru depozice. Druhá metoda využívá optické mikroskopie ve spojení s fázovým kontrastem. Výsledky obou metod byly porovnány a metody byly vyhodnoceny jako použitelné.

It seems to be very convenient to receive a medicine by inhalation instead of injection. Unfortunately transport of particles and targeted delivery of a drug in human respiratory airways is very complicated task. Therefore we carried out experiments and tested different methods for evaluation of particle deposition in a model of human lungs. The model included respiratory airways from oral cavity to 7th generation of branching. Particles were dispersed by TSI Small-scale Powder Disperser 3433 and delivered to the model. The model was disassembled into segments after the deposition of the particles and local deposition was measured. Two methods were used to analyse the samples, fluorescence spectroscopy and optical microscopy. The first method was based on measuring the intensity of luminescence, which represented the particle deposition. The second method used the optical microscope with phase-contrast objective. A dispersion of isopropanol and particles was filtrated using a vacuum filtration unit, a filter was placed on glass slide and made transparent. The particles on the filter were counted manually and the deposition was calculated afterwards. The results of the methods were compared and both methods proved to be useful.

Depozice aerosolu, optická mikroskopie, fluorescenční spektroskopie, dýchací cesty

Aerosol deposition, optical microscopy, fluorescent spectroscopy, human lungs

2013

25.03.2014

EDP Sciences

FRANCE

978-80-260-5375-0

2100-014X

EPJ Web of Conferences

67

1

82–85

4