Publication detail

Dynamical Deformation Compensation of Phase in Digital Holographic Microscopy

ZIKMUND, T. KVASNICA, L. LOVICAR, L. UHLÍŘOVÁ, H. CHMELÍK, R.

Czech title

Dynamická kompenzace deformace v digitální holografické mikroskopii

English title

Dynamical Deformation Compensation of Phase in Digital Holographic Microscopy

Type

conference paper

Language

en

Original abstract

Digital holographic microscopy (DHM) is a powerful tool for observation of biological samples. The main advantage of DHM imaging is the possibility to reconstruct the intensity image and the phase image of an observed sample in real-time. The background of phase images is deformed and this deformation is changing during a long-term experiment. We describe here a novel procedure of deformation compensation for sequence of phase images. This novel procedure is based on deformation compensation procedure for a single phase image which uses the least square method. The novel procedure is applicable to compensate deformation during observation in real-time. The proposed procedure is applied to phase images of cells obtained by a transmitted-light digital holographic microscope.

Czech abstract

Digitální holografická mikroskopie (DHM) je silný nástroj pro pozorování biologických vzorku. Hlavní výhodou DHM zobrazování je možnost rekonstruovat obraz intenzity a obraz fáze pozorovaného vzorku v realném case. Pozadí obrazu fáze je deformováno a tato deformace se mení behem dlouhodobého experimentu. My zde popisujeme novou proceduru kompenzace deformace pro sekvenci obrazu fáze. Tato nová procedura je založena na procedure kompenzující deformace na jediném obrazu fáze, která používá metodu nejmenších ctvercu. Tato procedura je aplikovatelná ke kompenzaci deformace behem pozorování v reálném case. Navržená procedura je použita na obraz fáze bunky obdržené transmisním digitálním holografickým mikroskopem.

English abstract

Digital holographic microscopy (DHM) is a powerful tool for observation of biological samples. The main advantage of DHM imaging is the possibility to reconstruct the intensity image and the phase image of an observed sample in real-time. The background of phase images is deformed and this deformation is changing during a long-term experiment. We describe here a novel procedure of deformation compensation for sequence of phase images. This novel procedure is based on deformation compensation procedure for a single phase image which uses the least square method. The novel procedure is applicable to compensate deformation during observation in real-time. The proposed procedure is applied to phase images of cells obtained by a transmitted-light digital holographic microscope.

Keywords in Czech

digitální holografický mikroskop, fázový obraz, kompenzace deformace, metoda nejmenších ctvercu

Keywords in English

digital holographic microscopy, phase image, deformation compensation, least square method

RIV year

2010

Released

14.12.2010

Publisher

SPIE

ISBN

9780819482365

ISSN

0277-786X

Book

Proceedings of SPIE, Volume 7746

Pages from–to

77460K-1–77460K-7

Pages count

7

BIBTEX


@inproceedings{BUT36565,
  author="Tomáš {Zikmund} and Lukáš {Kvasnica} and Luděk {Lovicar} and Hana {Uhlířová} and Radim {Chmelík},
  title="Dynamical Deformation Compensation of Phase in Digital Holographic Microscopy",
  booktitle="Proceedings of SPIE, Volume 7746",
  year="2010",
  month="December",
  pages="77460K-1--77460K-7",
  publisher="SPIE",
  isbn="9780819482365",
  issn="0277-786X"
}