Publication detail
Holographic Confocal Microscopy
CHMELÍK, R. HARNA, Z. MATOUŠEK, M.
English title
Holographic Confocal Microscopy
Type
conference paper
Language
en
Original abstract
Conventional confocal microscopes form the confocal image using various types of scanning systems. Besides other consequences of this fact, their maximum achievable imaging speed is limited. We report on the holographic confocal microscope which is based on a broad-spource image-plane holographic method adapted for a real-time reflected-light microscopy of the specimen surface regions. It is verified both theoretically and experimentally that the main imaging parameters of the microscope are comparable with those of a conventional confocal microscope. The only exception is the imaging speed which is not limited by any part of the optical system and thus can be much higher.
English abstract
Conventional confocal microscopes form the confocal image using various types of scanning systems. Besides other consequences of this fact, their maximum achievable imaging speed is limited. We report on the holographic confocal microscope which is based on a broad-spource image-plane holographic method adapted for a real-time reflected-light microscopy of the specimen surface regions. It is verified both theoretically and experimentally that the main imaging parameters of the microscope are comparable with those of a conventional confocal microscope. The only exception is the imaging speed which is not limited by any part of the optical system and thus can be much higher.
Keywords in English
confocal microscopy, holographic techniques
RIV year
2001
Released
01.01.1999
Publisher
EMBL, Heidelberg, Germany
Location
Heidelberg
Book
Focus on Microscopy 1999
Pages count
1
BIBTEX
@inproceedings{BUT3704,
author="Radim {Chmelík} and Zdeněk {Harna} and Miroslav {Matoušek},
title="Holographic Confocal Microscopy",
booktitle="Focus on Microscopy 1999",
year="1999",
month="January",
publisher="EMBL, Heidelberg, Germany",
address="Heidelberg"
}