Publication detail

Measurements of light scattering in an integrated microfluidic waveguide cytometer

SU, X. SINGH, K. CAPJACK, C. PETRÁČEK, J. BACKHOUSE, C.

Czech title

Měření rozptylu světla pomocí integrovaného mikrofluidního vlnovodného cytometru

English title

Measurements of light scattering in an integrated microfluidic waveguide cytometer

Type

journal article - other

Language

en

Original abstract

An integrated microfluidic planar optical waveguide system for measuring light scattered from a single scatterer is described. This system is used to obtain 2D side-scatter patterns from single polystyrene microbeads in a fluidic flow. Vertical fringes in the 2D scatter patterns are used to infer the location of the 90-deg scatter (polar angle). The 2D scatter patterns are shown to be symmetrical about the azimuth angle at 90 deg. Wide-angle comparisons between the experimental scatter patterns and Mie theory simulations are shown to be in good agreement. A method based on the Fourier transform analysis of the experimental and Mie simulation scatter patterns is developed for size differentiation.

Czech abstract

Je popisován systém založený na použití integrovaného mikrofluidního vlnovodného cytometru. Systém je použit k měření rozptylu světla a k získání dvojrozměrných roptylových obrazců. Experimentální data vykazují dobrý souhlas s teoretickými simulacemi založenými na Mieově teorii.

English abstract

An integrated microfluidic planar optical waveguide system for measuring light scattered from a single scatterer is described. This system is used to obtain 2D side-scatter patterns from single polystyrene microbeads in a fluidic flow. Vertical fringes in the 2D scatter patterns are used to infer the location of the 90-deg scatter (polar angle). The 2D scatter patterns are shown to be symmetrical about the azimuth angle at 90 deg. Wide-angle comparisons between the experimental scatter patterns and Mie theory simulations are shown to be in good agreement. A method based on the Fourier transform analysis of the experimental and Mie simulation scatter patterns is developed for size differentiation.

Keywords in Czech

rozptyl světla, cytometrie, analýza buněk, vlnovody, Mieova teorie

Keywords in English

light scattering, cytometry, cell analysis, waveguides, Mie theory

RIV year

2008

Released

01.03.2008

ISSN

1083-3668

Journal

JOURNAL OF BIOMEDICAL OPTICS

Volume

13

Number

2

Pages from–to

024024-1–024024-10

Pages count

10

BIBTEX


@article{BUT47015,
  author="Xuan-Tao {Su} and Kirat {Singh} and Clarence {Capjack} and Jiří {Petráček} and Christopher {Backhouse},
  title="Measurements of light scattering in an integrated microfluidic waveguide cytometer",
  journal="JOURNAL OF BIOMEDICAL OPTICS",
  year="2008",
  volume="13",
  number="2",
  month="March",
  pages="024024-1--024024-10",
  issn="1083-3668"
}