Publication detail

Measurement of Fiber Deposition in a Human Lung Model by Phase Contrast Microscopy with Automated Image Analysis

LÍZAL, F. ELCNER, J. BĚLKA, M. JEDELSKÝ, J. HOPKE, P. ŠTARHA, P. DRUCKMÜLLEROVÁ, H. JÍCHA, M.

Czech title

Měření depozice vláken v modelu lidských plic pomocí fázově kontrastní mikroskopie s automatickou analýzou obrazu

English title

Measurement of Fiber Deposition in a Human Lung Model by Phase Contrast Microscopy with Automated Image Analysis

Type

conference proceedings

Language

en

Original abstract

Deposition of fibers in human lungs is known as a health hazard. In-vitro measurements were performed with monodisperse glass fibers in a realistic model of human lungs up to seventh generation of branching to estimate the effect of fiber size and breathing pattern on fiber deposition. Deposited fibers were rinsed from the model segments and gathered on nitrocellulose filters. Phase-contrast microscopy with high resolution camera was used to capture images of filters with fibers. New software was developed for an automated image analysis and local deposition characteristics were calculated afterwards. The whole method proved to be a useful and valuable tool for the evaluation of fiber and particle deposition.

Czech abstract

Depozice vláken v lidských plicích je známým zdravotním rizikem. Byla provedena in-vitro měření s monodisperzními skelnými vlákny v realistickém modelu plic až do sedmé generace větvení s cílem odhadnout vliv velikosti vláken a způsobu dýchání na depozici vláken. Byla použita fázově kontrastní mikroskopie a kamera s vysokým rozlišením pro vytvoření snímků vláken na filtrech. Snímky byly analyzovány nově vytvořeným softwarem pro automatickou analýzu obrazu a na základě zjištěných počtů vláken na filtrech byly vypočteny depoziční charakteristiky.

English abstract

Deposition of fibers in human lungs is known as a health hazard. In-vitro measurements were performed with monodisperse glass fibers in a realistic model of human lungs up to seventh generation of branching to estimate the effect of fiber size and breathing pattern on fiber deposition. Deposited fibers were rinsed from the model segments and gathered on nitrocellulose filters. Phase-contrast microscopy with high resolution camera was used to capture images of filters with fibers. New software was developed for an automated image analysis and local deposition characteristics were calculated afterwards. The whole method proved to be a useful and valuable tool for the evaluation of fiber and particle deposition.

Keywords in Czech

usazování vláken, model lidských plic, fázově kontrastní mikroskop, analýza obrazu

Keywords in English

fiber deposition, human lung model, phase contrast microscopy, image analysis

Released

26.06.2012

Publisher

Vysoké učení technické v Brně

Location

Mikulov

ISBN

978-80-214-4529-1

Pages from–to

133–136

Pages count

4

BIBTEX


@proceedings{BUT95611,
  author="František {Lízal} and Jakub {Elcner} and Miloslav {Bělka} and Jan {Jedelský} and Philip K. {Hopke} and Pavel {Štarha} and Hana {Druckmüllerová} and Miroslav {Jícha},
  title="Measurement of Fiber Deposition in a Human Lung Model by Phase Contrast Microscopy with Automated Image Analysis",
  year="2012",
  month="June",
  pages="133--136",
  publisher="Vysoké učení technické v Brně",
  address="Mikulov",
  isbn="978-80-214-4529-1"
}