Publication detail

Surface Passivation Improves the Synthesis of Highly Stable and Specific DNA-Functionalized Gold Nanoparticles with Variable DNA Density

DEKA, J. MĚCH, R. IANESELLI, L. AMENITSCH, H. CACHO-NERIN, F. PARISSE, P. CASALIS, L.

Czech title

Pasivace povrchu zlepšuje syntézu vysoce stabilní a specificky DNA funkcionalizovaného povrchu Au nanočástic s proměnnou hustotou DNA

English title

Surface Passivation Improves the Synthesis of Highly Stable and Specific DNA-Functionalized Gold Nanoparticles with Variable DNA Density

Type

journal article in Web of Science

Language

en

Original abstract

We report a novel and multifaceted approach for the quick synthesis of highly stable single-stranded DNA (ssDNA) functionalized gold nanoparticles (AuNPs). The method is based on the combined effect of surface passivation by (1-mercaptoundec-11-yl)hexa(ethylene glycol) and low pH conditions, does not require any salt pretreatment or high excess of ssDNA, and can be generalized for oligonucleotides of any length or base sequence. The synthesized ssDNA-coated AuNPs conjugates are stable at salt concentrations as high as 3.0 M, and also functional and specific toward DNA–DNA hybridization, as shown from UV–vis spectrophotometry, scanning electron microscopy, gel electrophoresis, fluorescence, and small angle X-ray scattering based analyses. The method is highly flexible and shows an additional advantage of creating ssDNA-AuNP conjugates with a predefined number of ssDNA strands per particle. Its simplicity and tenability make it widely applicable to diverse biosensing applications involving ssDNA functionalized AuNPs.

Czech abstract

Článek se zabývá pasivací povrchu, která vede se zlepšení syntézy vysoce stabilní a specificky DNA funkcionalizovaného povrchu Au nanočástic s proměnnou hustotou DNA

English abstract

We report a novel and multifaceted approach for the quick synthesis of highly stable single-stranded DNA (ssDNA) functionalized gold nanoparticles (AuNPs). The method is based on the combined effect of surface passivation by (1-mercaptoundec-11-yl)hexa(ethylene glycol) and low pH conditions, does not require any salt pretreatment or high excess of ssDNA, and can be generalized for oligonucleotides of any length or base sequence. The synthesized ssDNA-coated AuNPs conjugates are stable at salt concentrations as high as 3.0 M, and also functional and specific toward DNA–DNA hybridization, as shown from UV–vis spectrophotometry, scanning electron microscopy, gel electrophoresis, fluorescence, and small angle X-ray scattering based analyses. The method is highly flexible and shows an additional advantage of creating ssDNA-AuNP conjugates with a predefined number of ssDNA strands per particle. Its simplicity and tenability make it widely applicable to diverse biosensing applications involving ssDNA functionalized AuNPs.

Keywords in Czech

DNA funktionalizace; Au nanočástice; pasivace povrchu

Keywords in English

DNA functionalization; gold nanoparticles; surface passivation; oligo ethylene glycol; salt-stability; specificity; variable DNA density

RIV year

2015

Released

10.03.2015

ISSN

1944-8252

Volume

7

Number

12

Pages from–to

7033–7040

Pages count

8

BIBTEX


@article{BUT114481,
  author="Jashmini {Deka} and Rostislav {Váňa} and Luca {Ianeselli} and Heinz {Amenitsch} and Fernando {Cacho-Nerin} and Pietro {Parisse} and Loredana {Casalis},
  title="Surface Passivation Improves the Synthesis of Highly Stable and Specific DNA-Functionalized Gold Nanoparticles with Variable DNA Density",
  year="2015",
  volume="7",
  number="12",
  month="March",
  pages="7033--7040",
  issn="1944-8252"
}