Detail publikace
Pasivace povrchu zlepšuje syntézu vysoce stabilní a specificky DNA funkcionalizovaného povrchu Au nanočástic s proměnnou hustotou DNA
DEKA, J. MĚCH, R. IANESELLI, L. AMENITSCH, H. CACHO-NERIN, F. PARISSE, P. CASALIS, L.
Český název
Pasivace povrchu zlepšuje syntézu vysoce stabilní a specificky DNA funkcionalizovaného povrchu Au nanočástic s proměnnou hustotou DNA
Anglický název
Surface Passivation Improves the Synthesis of Highly Stable and Specific DNA-Functionalized Gold Nanoparticles with Variable DNA Density
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
en
Originální abstrakt
We report a novel and multifaceted approach for the quick synthesis of highly stable single-stranded DNA (ssDNA) functionalized gold nanoparticles (AuNPs). The method is based on the combined effect of surface passivation by (1-mercaptoundec-11-yl)hexa(ethylene glycol) and low pH conditions, does not require any salt pretreatment or high excess of ssDNA, and can be generalized for oligonucleotides of any length or base sequence. The synthesized ssDNA-coated AuNPs conjugates are stable at salt concentrations as high as 3.0 M, and also functional and specific toward DNA–DNA hybridization, as shown from UV–vis spectrophotometry, scanning electron microscopy, gel electrophoresis, fluorescence, and small angle X-ray scattering based analyses. The method is highly flexible and shows an additional advantage of creating ssDNA-AuNP conjugates with a predefined number of ssDNA strands per particle. Its simplicity and tenability make it widely applicable to diverse biosensing applications involving ssDNA functionalized AuNPs.
Český abstrakt
Článek se zabývá pasivací povrchu, která vede se zlepšení syntézy vysoce stabilní a specificky DNA funkcionalizovaného povrchu Au nanočástic s proměnnou hustotou DNA
Anglický abstrakt
We report a novel and multifaceted approach for the quick synthesis of highly stable single-stranded DNA (ssDNA) functionalized gold nanoparticles (AuNPs). The method is based on the combined effect of surface passivation by (1-mercaptoundec-11-yl)hexa(ethylene glycol) and low pH conditions, does not require any salt pretreatment or high excess of ssDNA, and can be generalized for oligonucleotides of any length or base sequence. The synthesized ssDNA-coated AuNPs conjugates are stable at salt concentrations as high as 3.0 M, and also functional and specific toward DNA–DNA hybridization, as shown from UV–vis spectrophotometry, scanning electron microscopy, gel electrophoresis, fluorescence, and small angle X-ray scattering based analyses. The method is highly flexible and shows an additional advantage of creating ssDNA-AuNP conjugates with a predefined number of ssDNA strands per particle. Its simplicity and tenability make it widely applicable to diverse biosensing applications involving ssDNA functionalized AuNPs.
Klíčová slova česky
DNA funktionalizace; Au nanočástice; pasivace povrchu
Klíčová slova anglicky
DNA functionalization; gold nanoparticles; surface passivation; oligo ethylene glycol; salt-stability; specificity; variable DNA density
Rok RIV
2015
Vydáno
10.03.2015
ISSN
1944-8252
Ročník
7
Číslo
12
Strany od–do
7033–7040
Počet stran
8
BIBTEX
@article{BUT114481,
author="Jashmini {Deka} and Rostislav {Váňa} and Luca {Ianeselli} and Heinz {Amenitsch} and Fernando {Cacho-Nerin} and Pietro {Parisse} and Loredana {Casalis},
title="Surface Passivation Improves the Synthesis of Highly Stable and Specific DNA-Functionalized Gold Nanoparticles with Variable DNA Density",
year="2015",
volume="7",
number="12",
month="March",
pages="7033--7040",
issn="1944-8252"
}