Detail publikace

Mapování akumulace různých prvků v rostlinných tkání pomocí dvouenergiové rentgenové mikroradiografie na synchrotronu Dafne

REALE, L. KAISER, J. REALE, A. LAI, A. FLORA, F. BALERNA, A. CINQUE, G. FANELLI, M. RUGGIERI, F. FAENOV, A. PIKUZ, T. TUCCI, A. POMA, A. ZUPELLA, P. LIŠKA, M. MALINA, R.

Český název

Mapování akumulace různých prvků v rostlinných tkání pomocí dvouenergiové rentgenové mikroradiografie na synchrotronu Dafne

Anglický název

Mapping the intake of different elements in vegetal tissues by dual-energy X-ray imaging at DaFne synchrotron light source

Typ

článek v časopise - ostatní, Jost

Jazyk

en

Originální abstrakt

This article reports on the first utilization of the soft X-ray beamline at the DaFne synchrotron light source for mapping the intake of different elements in plant tissues. As a test, the method of dual-energy X-ray microradiography was applied to the investigation of the natural sulfur content in dried leaf and root samples. Our ultimate goal was to monitor the pollutant lead and its intake, which was added in controlled doses to the hydroponic medium of laboratory-controlled samples of vegetal species. The results obtained by the nondestructive X-ray radiographic analysis are compared to the values of concentrations determined by a standard chemical analysis utilizing atomic absorption spectroscopy. From this comparison the validity of the X-ray detection of heavy metals in biological samples has been confirmed. The superposition of the dual energy results on the simple planar radiography shows the representation of the pollutant intake directly on the sample structures. It should be pointed out that this method, developed here for plant root and leaves could be applied to any biological sample of interest, but the preparation and observation conditions necessitate different strategies according to the type of sample under analysis.

Český abstrakt

Článek pojednává o mapování akumulace různých prvků v rostlinných tkání pomocí dvouenergiové rentgenové mikroradiografie na synchrotronu Dafne.

Anglický abstrakt

This article reports on the first utilization of the soft X-ray beamline at the DaFne synchrotron light source for mapping the intake of different elements in plant tissues. As a test, the method of dual-energy X-ray microradiography was applied to the investigation of the natural sulfur content in dried leaf and root samples. Our ultimate goal was to monitor the pollutant lead and its intake, which was added in controlled doses to the hydroponic medium of laboratory-controlled samples of vegetal species. The results obtained by the nondestructive X-ray radiographic analysis are compared to the values of concentrations determined by a standard chemical analysis utilizing atomic absorption spectroscopy. From this comparison the validity of the X-ray detection of heavy metals in biological samples has been confirmed. The superposition of the dual energy results on the simple planar radiography shows the representation of the pollutant intake directly on the sample structures. It should be pointed out that this method, developed here for plant root and leaves could be applied to any biological sample of interest, but the preparation and observation conditions necessitate different strategies according to the type of sample under analysis.

Klíčová slova anglicky

dual energy X-ray analysis, intake, biodisposable, sulphur, lead

Rok RIV

2008

Vydáno

03.03.2008

ISSN

1059-910X

Časopis

Microscopy research and technique

Ročník

71

Číslo

3

Strany od–do

179–185

Počet stran

7

BIBTEX


@article{BUT45022,
  author="Jozef {Kaiser} and Miroslav {Liška} and Radomír {Malina},
  title="Mapping the intake of different elements in vegetal tissues by dual-energy X-ray imaging at DaFne synchrotron light source",
  journal="Microscopy research and technique",
  year="2008",
  volume="71",
  number="3",
  month="March",
  pages="179--185",
  issn="1059-910X"
}