Detail publikace
Určení rostlinných thiolů pomocí chromatografie a kolorimetrie v listech salátu
Vaclav Diopan, Violetta Shestivska, Ondrej Zitka, Michaela Galiova, Vojtech Adam, Jozef Kaiser, Ales Horna, Karel Novotny, Miroslav Liska, Ladislav Havel, Josef Zehnalek, Rene Kizek
Český název
Určení rostlinných thiolů pomocí chromatografie a kolorimetrie v listech salátu
Anglický název
Determination of Plant Thiols by Liquid Chromatography Coupled with Coulometric and Amperometric Detection in Lettuce Treated by Lead(II) Ions
Typ
článek v časopise - ostatní, Jost
Jazyk
en
Originální abstrakt
The main aim of this paper is to utilize high performance liquid chromatography with electrochemical detection for determination of thiols content in plants tissues of lettuce treated with lead(II) ions (0, 0.5 and 1 mM). We used two HPLC-ED instruments: HPLC coupled with one channel amperometric detector and HPLC coupled with twelve channel coulometric detector to detect simultaneously twelve thiols. The detection limits of thiols measured by CoulArray detector were about two magnitudes lower in comparison to those measured by Coulochem III detector and were from tens to hundreds pM. Under the optimal conditions, we utilized HPLC-CoulArray detector for analysis of tissues from lettuce plants. In addition, distribution and accumulation of lead ions with high spatial resolution was monitored using laser induced breakdown spectroscopy.
Český abstrakt
Článek pojednává o možnostech určení rostlinných thiolů pomocí chromatografie a kolorimetrie v listech salátu dopovaných olovem. Kromě chromatografie a kolorimetrie je diskutován i možnost použití spektrometrie laserem indukovaného mikroplazmatu (LIBS) na mapovaní rozložení olova v kontaminovaních rostlinách.
Anglický abstrakt
The main aim of this paper is to utilize high performance liquid chromatography with electrochemical detection for determination of thiols content in plants tissues of lettuce treated with lead(II) ions (0, 0.5 and 1 mM). We used two HPLC-ED instruments: HPLC coupled with one channel amperometric detector and HPLC coupled with twelve channel coulometric detector to detect simultaneously twelve thiols. The detection limits of thiols measured by CoulArray detector were about two magnitudes lower in comparison to those measured by Coulochem III detector and were from tens to hundreds pM. Under the optimal conditions, we utilized HPLC-CoulArray detector for analysis of tissues from lettuce plants. In addition, distribution and accumulation of lead ions with high spatial resolution was monitored using laser induced breakdown spectroscopy.
Klíčová slova anglicky
Thiols, Liquid chromatography, Coulometric detection, Amperometric detection, Heavy metal, Laser induced breakdown spectroscopy
Rok RIV
2010
Vydáno
12.01.2010
ISSN
1040-0397
Časopis
ELECTROANALYSIS
Ročník
22
Číslo
11
Strany od–do
1248–1259
Počet stran
12
BIBTEX
@article{BUT49960,
author="Jozef {Kaiser} and Miroslav {Liška} and Michaela {Vašinová Galiová},
title="Determination of Plant Thiols by Liquid Chromatography Coupled with Coulometric and Amperometric Detection in Lettuce Treated by Lead(II) Ions",
journal="ELECTROANALYSIS",
year="2010",
volume="22",
number="11",
month="January",
pages="1248--1259",
issn="1040-0397"
}